reported (Johnson et al. 2007). However, NRSE in CART gene promoter has not been predicted. In view of the function of NRSF–NRSE system and the distribution proﬁle of CART, we postulate that NRSF may play a regulatory role in the speciﬁc expression of CART gene. In this study, we identiﬁed a conserved NRSE sequence in

The human AVP NRSE-like motif. a, the human AVP promoter is shown in the center, aligned to the NRSE consensus sequence (12) above, and a motif from the

They noted that the SCG10 regulatory region contains both activation and repression (i.e., silencer) domains and that similar NRSE-like sequence elements have been identified in other neuron-specific genes. NRSE sequences were effective in restricting expression of bipartite Gal4-based ‘driver’ transgenes within the context of an enhancer trap and when associated with a defined promoter and enhancer. However, NRSE sequences did not serve to restrict expression of an upstream activating sequence (UAS)-based reporter/effector 2001-11-01 · Sequence analysis of this promoter region revealed the presence of a neuron-restrictive silencer element (NRSE) known to bind repressor zinc finger protein REST. This factor is not expressed in insulin-secreting and neuron-like cells. NRSE is a regulator y sequence that is present in several neuronal genes (8) and that was, up to now, thought to silence neuronal gene transcription in nonneuronal cells (6, 7, 9 –14).

In non-neuronal cells, neuron-restrictive silencer factor (NSRF) actively represses gene transcription via a sequence-specific DNA motif known as the neuron-restrictive silencer element (NRSE). This DNA motif has been identified in many genes that are specific markers for cells of neuronal and neuroendocrine lineage. We found that the mouse Na v 1.8 gene contains two putative conserved NRSE sites, a forward-oriented sequence within the 5′-untranslated region (NRSE-1) and a reverse-oriented sequence within intron 10 (NRSE-2) (Fig. 1 B, C). To better restrict transgene expression to the nervous system in zebrafish larvae, we have used DNA sequences derived from the neuron-restrictive silencing element (NRSE).

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NRSF, a zinc finger transcription factor that binds to the 21-bp recognition sequence NRSE, is a transcriptional repressor of multiple neuronal genes and is normally down-regulated upon neuronal differentiation (44. Su X. To address this, we investigated the effect of REST/NRSF and REST4 on the activity-dependent activation of BDNF gene promoter I (BDNFp-I) using cultured rat cortical neurons.

We found that the mouse Na v 1.8 gene contains two putative conserved NRSE sites, a forward-oriented sequence within the 5′-untranslated region (NRSE-1) and a reverse-oriented sequence within intron 10 (NRSE-2) (Fig. 1 B, C).

As shown in Fig. 2b, REST bound to the mouse Bsx NRSE by forming stable DNA–protein complexes (lane 2). Consensus sequence logos for NRSE motifs associated with protein-coding genes and in gene-distant regions are similar. NRSE motifs associated with protein-coding genes were located ≤ 100 kb distance. The NRSEs in GD were located > 100 kb from a protein-coding gene. Additional file 3: Table S3. (43K, xlsx) canonical RE1/NRSE sites, which have a variable linker between the two half-site RE1s/NRSEs,7 while a third type features half-site RE1/NRSE with only one-half of the canonical RE1/NRSE.7 Of note, variations of the RE1/NRSE sequence are associated with modulation of REST/NRSF affinity to the respective DNA site, allowing some RE1/NRSE sites 2006-09-26 Plasmid pAAV-DR1-NRSE-RFP from Dr. Edward Callaway's lab contains the insert peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV and is published in Front Neural Circuits.

2007-06-08 The only likely repressor sequence was NRSE, which interacts with the silencer NRSF. NRSF, a zinc finger transcription factor that binds to the 21-bp recognition sequence NRSE, is a transcriptional repressor of multiple neuronal genes and is normally down-regulated upon neuronal differentiation (44.
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Of these, 892, 944, and 291 are found in the human, mouse, and Fugu genomes, respectively. The frequency at which each RE1 variation occurred in the human genome was compared to the frequency in the mouse and Fugu genomes (see Table 1). The NRSE-TK-luc and mutNRSE-TK-luc series were made by introducing a fragment of the minimal thymidine kinase (TK) promoter and double stranded oligonucleotides containing the NRSE or mutant NRSE sites from the BNP promoter into pGL4.10 (Promega). Certified flight nurses monitor and administer life-sustaining care to patients during air transport. Because of the unpredictability of this type of transportation and the absence of large support teams, CFRNs must work independently to address issues that can surface during flight.

METHODS Genomic library A Lamda FIXII mouse genomic library (Stratagene, La Jolla, CA) The identification of a common cis -acting silencer element, a neuron-restrictive silencer element (NRSE), in multiple neuron-specific genes, together with the finding that zinc finger transcription factor REST/NRSF/XBR could confer NRSE-mediated silencing in non-neuronal cells, suggested that REST/NRSF/XBR is a master negative regulator of neurogenesis. NRSE sequence in the HCN1 gene and the structure of the NRSF gene are conserved in humans, mice, and rats [8], as shown in Figure 1. FIGURE 1: HCN1 Gene Contains a Conserved NRSE HCN1 Gene Contains a Conserved NRSE (McClelland, et al., 2008) Aside from the NRSE consensus sequence (the series of nucleotides most frequently found at NRSE 3700J *** Recommended to Fulfill Tier I Jr. Comp NRSE 3700J*** Recommended to Fulfill Tier I Jr. Comp NRSE 4741**** 2nd School Nurse Course - must be taken in sequence *NRSE 4600, Nursing Excellence – contact your advisor via email to request permission to be added to the course at least 5 weeks prior to start date. Nov 27, 2006 Herein, we identify a conserved GC sequence next to NRSE region in the mouse MOR gene.
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In non-neuronal cells, neuron-restrictive silencer factor (NSRF) actively represses gene transcription via a sequence-specific DNA motif known as the neuron-restrictive silencer element (NRSE). This DNA motif has been identified in many genes that are specific markers for cells of neuronal and neuroendocrine lineage.

We find that one such sequence, REx2, when used in conjunction with several basal promoters, robustly suppresses transgene expression in non-neuronal tissues. The sequence analysis of HCN4 gene revealed the presence of a conserved NRSE motif, which is known to bind the transcriptional factor neuron-restrictive silencing factor (NRSF). A promoter analysis of HCN4 with rat cardiac myocytes identified the region inducing a basal transcriptional activity. The RE1 consensus sequence used in this study is degenerate, allowing a total of 4,096 permutations. Of these, 892, 944, and 291 are found in the human, mouse, and Fugu genomes, respectively. The frequency at which each RE1 variation occurred in the human genome was compared to the frequency in the mouse and Fugu genomes (see Table 1). The NRSE-TK-luc and mutNRSE-TK-luc series were made by introducing a fragment of the minimal thymidine kinase (TK) promoter and double stranded oligonucleotides containing the NRSE or mutant NRSE sites from the BNP promoter into pGL4.10 (Promega).